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Assay formats and screening

Assay development capabilities

Polyphor has in-house expertise in assay development (primary and secondary assays) for a wide range of target classes, which are available for internal and collaborative projects. These target classes include:  

  • Protein-Protein Interactions (PPIs)
  • G-coupled protein receptors
  • Tyrosine receptor kinases
  • Wnt pathway
  • Proteases
  • Ion channels
  • Toll-like receptors
  • Antimicrobials

The current screening assay formats include:

  • Calcium Flux, β-arrestin and ligand binding for GPCR assays
  • Chemotaxis and cell migration
  • Fluorescent (FP, FRET), luminescence and colorimetric assays
  • FLIPR screen
  • Cell growth, proliferation and apoptosis assays
  • Luminex multiplexed technology
  • Enzyme linked immunosorbent assays (ELISAs)
  • Phage Display screening
  • Biacore assays for protein, peptide and small molecule or fragment interaction
  • Antibacterial compound profiling: in vitro susceptibility, killing time curves, biofilms, membrane lysis, in vitro resistance

Molecular biology capabilities include:

Cell transfection, transformation and transduction, cloning, protein expression and purification.

 

 

Polyphor Ltd
Hegenheimermattweg 125
CH-4123 Allschwil
Switzerland
Tel. +41 61 567 16 00
Fax +41 61 567 16 01
info@polyphor.com

Last modified:   02.04.2013

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